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Subject and approach of our problem:
We were interested in the benefits of coconut oil.
Coconut oil would be used in two fields, in food industry with antioxidant properties thanks to its vitamins A and E or in cosmetics with moisturizing and nourishing properties on our skin.
However, following the research we did on coconut oil, we discovered that it would also be used for oral hygiene with its antibacterial properties. Furthering this research we discovered the "oïl pulling" technique.
This technique is an ancestral therapy that reveals ayurvedic medicine, so it would be a natural alternative to clean teeth, it could replace a usual mouthwash where bacteria get carried away and dissolve them in oil.
Explanation of the oïl pulling technique :
Rinse your mouth with lukewarm water
Take a tablespoon of coconut oil.
Rotate the coconut oil in your mouth for 15 minutes.
Spit and then rinse your mouth well
Brushing your teeth
We therefore decided to focus our problem on this new property that was unknown, that is to say on the inhibitory power that this oil would have in contact with bacteria in our mouth.
Our experiments will allow us to know if coconut oil would have an inhibiting power with the bacteria in our mouth.
-Our test sessions:
first test session:
During this first test session we performed three manipulations.
-Qualitative analysis of our products, i.e. toothpaste, mouthwash and coconut oil after their use in our mouth on trypticase soy agar to control their purity.
-A quantitative analysis with a count before and after the use of each product, with a student associated with a product to determine their concentration in the mouth and demonstrate the effectiveness of the product to be tested.
-Observation of the types of microorganisms present in our mouths on 4 agars, Chapman, Sabouraud, Trypticase Soy Agar and Blood Agar in order to compare which dental cleaning technique is the most effective on oral bacteria.
Second test session:
-MIC with three types of microorganisms (Candida Albican, Staphylococcus Aureus and E. Coli) in coconut oil to determine the minimum concentration of coconut oil on 3 different strains. This MIC could potentially prove that coconut oil inhibits the culture of a bacterial strain of the oral flora and that coconut oil would therefore have inhibitory properties.
-Test of coconut oil inhibition on different media (Chapman and Sabouraud) and different microorganisms (Candida Albican, Staphylococcus Aureus and E. Coli) by comparing two cans on the same medium but a different microorganism proving the inhibition power of coconut oil.
Group sessions :
-An other MIC with the same microorganism but in a more soluble medium for coconut oil, i.e. LT100, in order to determine the minimum concentration of coconut oil on 3 different strains. This MIC could potentially prove that coconut oil inhibits the culture of a bacterial strain of the oral flora and that coconut oil would therefore have inhibitory properties.
-The bactericidal activity of coconut oil by dilution-neutralization method
-Verification of microorganisms in coconut oil and a study of the growth of E. Coli in the presence of coconut oil.
Interpretation of results :
first test session:
-For the purity control on our products, we can see that after their use there is always a low presence of microorganisms but however coconut oil shows advantages, so we can conclude that mouthwash and toothbrush are of pure advantage but not coconut oil.
-Coconut oil does not destroy oral bacteria and is currently not effective compared to a mouthwash and toothbrush that removes oral bacteria.
-For the observation of the types of microorganisms present in our mouth we cannot know the name of each microorganism present in our mouth.
Second test session:
-For ICM, we had chosen a medium that was not diluted well with coconut oil as it is not soluble in water but with fatty acids. We cannot know the minimum inhibition concentration.
-Test of inhibition of coconut oil on different media (Chapman and Sabouraud) and different microorganisms (Candida Albican, Staphylococcus Aureus and E. Coli) coconut oil does not inhibit bacteria.
Group sessions :
-For MIC in LT100 medium, the bacterial concentration was the same for each tube, we cannot determine its minimal inhibitory concentration.
-For the bactericidal activity since there were many bacteria in the dilution-neutralization, we can therefore say that coconut oil could not work.
-Verification of the microorganisms present in the coconut oil, the results are the same as the first time there are microorganisms in the coconut oil.
Conclusion:
Following our experiments in comparison with a mouthwash and a usual toothpaste, we can thus affirm that coconut oil has no effectiveness against the bacteria of our mouth with a first treatment with Oïl pulling and thus does not have inhibiting properties.
